Genetic Relationships Among Medicago sativa L. Clones Commonly Grown in Central Anatolia | Zendy

ONARICI Selma GÜLBİTTİ KENDİR | Zendy

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Genetic Relationships Among Medicago sativa L. Clones Commonly Grown in Central Anatolia

Author(s) -

ONARICI Selma GÜLBİTTİ KENDİR

Publication year - 2009

Publication title -

tarım bilimleri dergisi

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.195

H-Index - 16

eISSN - 2148-9297

pISSN - 1300-7580

DOI - 10.1501/tarimbil_0000001084

Subject(s) - amplified fragment length polymorphism , ecori , biology , primer (cosmetics) , microbiology and biotechnology , medicago sativa , physics , botany , genetics , restriction enzyme , dna , medicine , genetic diversity , population , environmental health , thermodynamics

Amplified fragment length polymorphism (AFLP) markers were used to investigate genotypic variability among 34 alfalfa clones (M. sativa) using 15 primer combinations with restriction enzymes EcoRI, PstI and MseI. 34 unique AFLP fragments were observed. The 15 primer pairs produced a total of 1002 fragments of which 460 were polymorphic. The number of polymorphic fragments detected per primer combination ranged 7 to 67. Furthermore, 22 clone-specific markers were also detected in the 13 clones. Data analysis was performed with NTSYSpc version 2.1 software. Genetic distance values ranged 5.9374 to 1.1453. Fifteen clones which showed the highest genetic variation were selected for producing synthetic variety of Alfalfa.

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