Yonca (Medicago sativa L.)’da Somatik Embriyogenesis Aracılığıyla Bitki Rejenerasyonu

Alfalfa is one of the world’s most productive forage crops and has wide adaptation because of highly nutritious fodder for farm animals. Regeneration capacity of various explants of cv. verco using various protocols was studied to evaluate their effects. Five explants (hypocotyl, cotyledon, stem, leaf and petiole) were cultured to evaulate two protocols. An interaction was observed between the source of explants and applied protocols. The explant were cultured on B5h medium containing 1 mg/l adenine, 1 mg/l 2,4-D and 0.2 mg/l kinetin in the first protocol. After 21 days the regenerating calli were first subcultured in SH medium containing 11 mg/l 2,4-D and 2 mg/l kinetin. Subsequently they were transferred to Boi2y medium. Developing embryonic culusters were transferred to MSO medium for recovery. The highest number of 78 somatic embryos were obtained from hypocotly. In the 2nd protocol, the explants were cultured in B5h medium containing 4 mg/l 2,4-D for 8 weeks initially and then moved to plant growth regulator free B5h medium. The plants were recovered from embryos in MSO medium. The highest number of 94 embryos were obtained from leaf explants.