Alpha-fetoprotein genosensor based on quartz crystal microbalance | Zendy

DURKUT Serap ŞEKER | Zendy

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Alpha-fetoprotein genosensor based on quartz crystal microbalance

Author(s) -

DURKUT Serap ŞEKER

Publication year - 2016

Publication title -

communications faculty of science university of ankara series b chemistry and chemical engineering

Language(s) - English

Resource type - Journals

ISSN - 1303-6017

DOI - 10.1501/commub_0000000554

Subject(s) - quartz crystal microbalance , glutaraldehyde , oligonucleotide , quartz , atomic force microscopy , materials science , chemistry , crystal (programming language) , nanotechnology , chromatography , gene , biochemistry , adsorption , organic chemistry , composite material , computer science , programming language

In this study, a genosensor based on quartz crystal microbalance (QCM) has been developed for the detection of single-stranded oligonucleotide specific to alpha-fetoprotein (AFP) gene. The quartz crystals were coated with polyethyleneimine (PEI) and then crosslinked with glutaraldehyde (GA). The oligonucleotide probe was immobilized onto the PEI-GA film. The coatings were characterized by using atomic force microscopy. Hybridization reaction between probe and the complementary strand was monitored by the QCM system in real-time. This work demonstrated that QCM is a suitable tool for the real-time monitoring of hybridization on the crystal surface without using any label.

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