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Nanoluciferase (NanoLuc) is a newly developed small monomeric luciferase reporter with the brightest bioluminescence reported to date. Recently, we have used NanoLuc as a novel ultrasensitive bioluminescent probe for ligand-receptor interaction studies. In the present highlight, I discuss its general application in ligand-receptor interactions and other binding studies. This novel ultrasensitive bioluminescent probe will facilitate a wide range of biological studies as its new applications are continuously developed.

Using the newly developed nanoluciferase as an ultrasensitive bioluminescent probe for ligand-receptor interaction studies