English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

There are between 20 and 30 drugs available for the treatment of seizures but despite this ~30% of the epilepsy population remains unresponsive to treatment. The underlying mechanisms behind drug resistance in temporal lobe epilepsy (TLE) have not been completely identied. The purpose of this study was to determine if distinct types of neuronal firing patterns occurring in human epileptic cortex are altered by carbamazepine (CBZ). We used whole-cell patch-clamp techniques combined with intracellular labeling to electrophysiologically and morphologically characterize neuronal populations in resected cortical tissue from patients with drug resistant epilepsy. We then determined if cells were uniformly resistant to carbamazepine or whether only a subset did not respond. Cortical spiking patterns were segregated in six main clusters: adapting high frequency cluster 1 and 2 (AHF1 and AHF2), adapting low frequency cluster 1 and 2 (ALF1 and ALF2), strongly adapting low frequency group (sALF) and one spike cluster (OS). A morphological analysis showed that some spiking patterns tend to be associated with specific neuronal morphology. OS group included only pyramidal cells while adapting high frequency groups (AHF1 &AHF2) displayed typical interneuron phenotype. Finally, we found that CBZ does not uniformly suppress neuronal activity as only ~27% of interneurons and ~40% of pyramidal cells were carbamazepine insensitive. These data indicate that in humans with DRE there is a heterogeneous CBZ insensitivity in all sub-populations of neurons.

Electrophysiological properties and carbamazepine sensitivity of epileptic human cortical neurons