Application of Random Amplified Polymorphic DNA Markers for Genetic Diversity Assessment of Pomegranate (Punica granatum L.) Cultivars in Duhok Governorate – Kurdistan of Iraq | Zendy

Dalal Sinjare | Zendy; Sabrya Zubair | Zendy; Avesta Ali | Zendy; Jaladet Jubrael | Zendy

Open Access

Application of Random Amplified Polymorphic DNA Markers for Genetic Diversity Assessment of Pomegranate (Punica granatum L.) Cultivars in Duhok Governorate – Kurdistan of Iraq

Author(s) -

Dalal Sinjare,

Sabrya Zubair,

Avesta Ali,

Jaladet Jubrael

Publication year - 2016

Publication title -

aro-the scientific journal of koya university

Language(s) - English

Resource type - Journals

eISSN - 2410-9355

pISSN - 2307-549X

DOI - 10.14500/aro.10078

Subject(s) - rapd , genetic diversity , cultivar , punica , veterinary medicine , biology , horticulture , botany , medicine , population , environmental health

Random amplified polymorphic DNA (RAPD) markers were used to investigate the genetic diversity among 13 cultivars of pomegranate cultivated in Duhok governorate, Kurdistan region/Iraq. Unweighed pairgroup method average clustering divided the 13 cultivars into three main groups. In RAPD analysis, 12 out of 30 employed random primers showed good amplification and polymorphism on pomegranate samples with a total of 107 bands. The percentage of polymorphic DNA bands ranged from 66.6% (OPX-17) to 100% (OPO-07, OPS-17, and OPE-18, OPP-02, OPK-08, OPL-17, and OPR-01) with an average of polymorphic rate of 94.39. According to matrix coefficient, the lowest genetic similarity was observed between Soormiz and Rash Dendikheer (0.1065). The Dereger and Kapepan populations were most similar ones with coefficient of 0.9374.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research