Evaluation of DNA Extraction Methods of Mule Dung | Zendy

Rajesh Kumar Vaid | Zendy; Taruna Anand | Zendy; Priyanka Batra | Zendy; Ram Avtar Legha | Zendy; Bhupendra Nath Tripathi | Zendy

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Evaluation of DNA Extraction Methods of Mule Dung

Author(s) -

Rajesh Kumar Vaid,

Taruna Anand,

Priyanka Batra,

Ram Avtar Legha,

Bhupendra Nath Tripathi

Publication year - 2019

Publication title -

defence life science journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.135

H-Index - 3

eISSN - 2456-379X

pISSN - 2456-0537

DOI - 10.14429/dlsj.4.14269

Subject(s) - dna extraction , isolation (microbiology) , cow dung , biology , dna , extraction (chemistry) , microbial population biology , microbiology and biotechnology , ecology , bacteria , polymerase chain reaction , chromatography , chemistry , genetics , gene , fertilizer

DNA isolation is a critical step in microbial community analysis of animal dung. DNA isolation from mule dung is challenging due to microbial diversity, composition and chemical nature of mule dung. Therefore, selection of an appropriate DNA isolation method is important to analyse the complete microbial diversity. In the current study, we evaluated the DNA isolation from mule dung samples (n=11) using QiAmp Mini stool kit as per manufacturer’s procedure with modifications. The results suggest that modifications in proprietary column based method improved the DNA quality and quantity suitable for mule dung microbial community analyses.

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