A novel approach for the assembly of complex genomic DNA cloned into bacterial artificial chromosome vectors: assembly and analysis of Triticum aestivum chromosome arm 7DS | Zendy

Paul Visendi Muhindira | Zendy

Open Access

A novel approach for the assembly of complex genomic DNA cloned into bacterial artificial chromosome vectors: assembly and analysis of Triticum aestivum chromosome arm 7DS

Author(s) -

Paul Visendi Muhindira

Publication year - 2016

Publication title -

queensland's institutional digital repository (the university of queensland)

Language(s) - English

Resource type - Dissertations/theses

DOI - 10.14264/uql.2016.521

Subject(s) - shotgun sequencing , sequence assembly , bacterial artificial chromosome , genome , computational biology , biology , hybrid genome assembly , dna sequencing , shotgun , chromosome , genetics , genome project , reference genome , whole genome sequencing , gene , transcriptome , gene expression

Next generation DNA sequencing technologies have led to an exponential growth in the number of genomes being sequenced. While generating whole genome shotgun (WGS) assemblies using next generation sequencing (NGS) is relatively fast and inexpensive, the application of this approach to the assembly of highly repetitive and complex genomes such as wheat results in inferior assemblies thus slowing efforts in identifying markers for

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