Variability of the Reverse Transcription Step: Practical Implications | Zendy

Stephen A. Bustin | Zendy; Harvinder S. Dhillon | Zendy; Sara Kirvell | Zendy; Christina Greenwood | Zendy; Michael Parker | Zendy; Gregory L. Shipley | Zendy; Tania Nolan | Zendy

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Variability of the Reverse Transcription Step: Practical Implications

Author(s) -

Stephen A. Bustin,

Harvinder S. Dhillon,

Sara Kirvell,

Christina Greenwood,

Michael Parker,

Gregory L. Shipley,

Tania Nolan

Publication year - 2014

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2014.230615

Subject(s) - reverse transcriptase , complementary dna , rna , real time polymerase chain reaction , reverse transcription polymerase chain reaction , messenger rna , biology , computational biology , reproducibility , microbiology and biotechnology , transcription (linguistics) , chemistry , genetics , gene , chromatography , linguistics , philosophy

The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency is known to be variable, little attention has been paid to the practical implications of that variability.

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