Open AccessRapid Electrokinetic Isolation of Cancer-Related Circulating Cell-Free DNA Directly from Blood
Author(s) -
Avery Sonnenberg,
Jennifer Y. Marciniak,
Laura Z. Rassenti,
Emanuela M. Ghia,
Elaine A. Skowronski,
Sareh Manouchehri,
James P. McCanna,
George F. Widhopf,
Thomas J. Kipps,
Michael J. Heller
Publication year - 2013
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2013.214874
Subject(s) - liquid biopsy , dna , ighv@ , microbiology and biotechnology , cell free fetal dna , digital polymerase chain reaction , dna sequencer , polymerase chain reaction , whole blood , cold pcr , biomarker , amplicon , biology , chronic lymphocytic leukemia , dna extraction , cancer , dna sequencing , gene , point mutation , leukemia , immunology , genetics , mutation , pregnancy , fetus , prenatal diagnosis
Circulating cell-free DNA (ccf-DNA) is becoming an important biomarker for cancer diagnostics and therapy monitoring. The isolation of ccf-DNA from plasma as a "liquid biopsy" may begin to replace more invasive tissue biopsies for the detection and analysis of cancer-related mutations. Conventional methods for the isolation of ccf-DNA from plasma are costly, time-consuming, and complex, preventing the use of ccf-DNA biomarkers for point-of-care diagnostics and limiting other biomedical research applications.
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