Digital Droplet PCR for Rapid Quantification of Donor DNA in the Circulation of Transplant Recipients as a Potential Universal Biomarker of Graft Injury | Zendy

Julia Beck | Zendy; Sarah Bierau | Zendy; Stefan Balzer | Zendy; Reiner Andag | Zendy; Philipp Kanzow | Zendy; Jessica Schmitz | Zendy; Jochen Gaedcke | Zendy; Onnen Moerer | Zendy; Jan E. Slotta | Zendy; Philip D. Walson | Zendy; Otto Kollmar | Zendy; Michael Oellerich | Zendy; Ekkehard Schütz | Zendy

Open Access

Digital Droplet PCR for Rapid Quantification of Donor DNA in the Circulation of Transplant Recipients as a Potential Universal Biomarker of Graft Injury

Author(s) -

Julia Beck,

Sarah Bierau,

Stefan Balzer,

Reiner Andag,

Philipp Kanzow,

Jessica Schmitz,

Jochen Gaedcke,

Onnen Moerer,

Jan E. Slotta,

Philip D. Walson,

Otto Kollmar,

Michael Oellerich,

Ekkehard Schütz

Publication year - 2013

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2013.210328

Subject(s) - digital polymerase chain reaction , biomarker , transplantation , medicine , cell free fetal dna , biopsy , gastroenterology , polymerase chain reaction , biology , gene , pregnancy , biochemistry , fetus , genetics , prenatal diagnosis

Cell-free DNA (cfDNA) from grafts in the circulation of transplant recipients is a potential biomarker of rejection. Its usefulness was investigated after heart transplantation during the maintenance phase by use of microarrays and massive parallel sequencing of donor and recipient DNA. Disadvantages of these methods are high costs, long turnaround times, and need for donor DNA. Therefore, we sought to develop a rapid and cost-effective method using digital droplet PCR (ddPCR).

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