Droplet Digital PCR Measurement of HER2 Copy Number Alteration in Formalin-Fixed Paraffin-Embedded Breast Carcinoma Tissue | Zendy

Phillip Belgrader | Zendy; Stephanie Tanner | Zendy; John F. Regan | Zendy; Ryan Koehler | Zendy; Benjamin J. Hindson | Zendy; Alexandra S. Brown | Zendy

Open Access

Droplet Digital PCR Measurement of HER2 Copy Number Alteration in Formalin-Fixed Paraffin-Embedded Breast Carcinoma Tissue

Author(s) -

Phillip Belgrader,

Stephanie Tanner,

John F. Regan,

Ryan Koehler,

Benjamin J. Hindson,

Alexandra S. Brown

Publication year - 2013

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2012.197855

Subject(s) - immunohistochemistry , digital polymerase chain reaction , fluorescence in situ hybridization , biology , breast carcinoma , breast cancer , concordance , pathology , fish <actinopterygii> , carcinoma , oncogene , microbiology and biotechnology , polymerase chain reaction , cancer , medicine , gene , chromosome , bioinformatics , cell cycle , genetics , fishery

Human epidermal growth factor receptor 2 (HER2) testing is routinely performed by immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH) analyses for all new cases of invasive breast carcinoma. IHC is easier to perform, but analysis can be subjective and variable. FISH offers better diagnostic accuracy and added confidence, particularly when it is used to supplement weak IHC signals, but it is more labor intensive and costly than IHC. We examined the performance of droplet digital PCR (ddPCR) as a more precise and less subjective alternative for quantifying HER2 DNA amplification.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research