Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry | Zendy

Ming-Luan Chen | Zendy; Fan Shen | Zendy; Wei Huang | Zendy; Jiahui Qi | Zendy; Yinsheng Wang | Zendy; YuQi Feng | Zendy; Song-Mei Liu | Zendy; BiFeng Yuan | Zendy

Open Access

Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry

Author(s) -

Ming-Luan Chen,

Fan Shen,

Wei Huang,

Jiahui Qi,

Yinsheng Wang,

YuQi Feng,

Song-Mei Liu,

BiFeng Yuan

Publication year - 2013

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2012.193938

Subject(s) - 5 hydroxymethylcytosine , hepatocellular carcinoma , epigenetics , chemistry , genomic dna , dna , detection limit , microbiology and biotechnology , liver cancer , tandem mass spectrometry , chromatography , dna methylation , mass spectrometry , biology , cancer research , biochemistry , gene , gene expression

5-Methylcytosine (5-mC) is an important epigenetic modification involved in development and is frequently altered in cancer. 5-mC can be enzymatically converted to 5-hydroxymethylcytosine (5-hmC). 5-hmC modifications are known to be prevalent in DNA of embryonic stem cells and neurons, but the distribution of 5-hmC in human liver tumor and matched control tissues has not been rigorously explored.

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