Multiplex Picoliter-Droplet Digital PCR for Quantitative Assessment of DNA Integrity in Clinical Samples | Zendy

Audrey Didelot | Zendy; Steve K. Kotsopoulos | Zendy; Audrey Lupo | Zendy; Deniz Pekin | Zendy; Xinyu Li | Zendy; Ivan Atochin | Zendy; Preethi Srinivasan | Zendy; Qun Zhong | Zendy; Jeff Olson | Zendy; Darren R. Link | Zendy; Pierre LaurentPuig | Zendy; Hélène Blons | Zendy; J. Brian Hutchison | Zendy; Valérie Taly | Zendy

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Multiplex Picoliter-Droplet Digital PCR for Quantitative Assessment of DNA Integrity in Clinical Samples

Author(s) -

Audrey Didelot,

Steve K. Kotsopoulos,

Audrey Lupo,

Deniz Pekin,

Xinyu Li,

Ivan Atochin,

Preethi Srinivasan,

Qun Zhong,

Jeff Olson,

Darren R. Link,

Pierre LaurentPuig,

Hélène Blons,

J. Brian Hutchison,

Valérie Taly

Publication year - 2013

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2012.193409

Subject(s) - digital polymerase chain reaction , multiplex , dna , dna sequencing , biology , genomic dna , dna nanoball sequencing , multiplex polymerase chain reaction , genotyping , microbiology and biotechnology , polymerase chain reaction , computational biology , genomic library , genetics , gene , genotype , base sequence

Assessment of DNA integrity and quantity remains a bottleneck for high-throughput molecular genotyping technologies, including next-generation sequencing. In particular, DNA extracted from paraffin-embedded tissues, a major potential source of tumor DNA, varies widely in quality, leading to unpredictable sequencing data. We describe a picoliter droplet-based digital PCR method that enables simultaneous detection of DNA integrity and the quantity of amplifiable DNA.

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