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Reference Measurement Procedure for Total Glycerides by Isotope Dilution GC-MS
Author(s) -
Selvin H. Edwards,
Shelton L Stribling,
Susan D Pyatt,
Mary M. Kimberly
Publication year - 2012
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2011.177063
Subject(s) - isotope dilution , glyceride , chromatography , chemistry , dilution , gas chromatography–mass spectrometry , mass spectrometry , organic chemistry , physics , fatty acid , thermodynamics
The CDC's Lipid Standardization Program established the chromotropic acid (CA) reference measurement procedure (RMP) as the accuracy base for standardization and metrological traceability for triglyceride testing. The CA RMP has several disadvantages, including lack of ruggedness. It uses obsolete instrumentation and hazardous reagents. To overcome these problems the CDC developed an isotope dilution GC-MS (ID-GC-MS) RMP for total glycerides in serum.

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