Snapback Primer Genotyping of the Gilbert Syndrome UGT1A1 (TA)n Promoter Polymorphism by High-Resolution Melting | Zendy

Jared S. Farrar | Zendy; Robert Palais | Zendy; Carl T. Wittwer | Zendy

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Snapback Primer Genotyping of the Gilbert Syndrome UGT1A1 (TA)n Promoter Polymorphism by High-Resolution Melting

Author(s) -

Jared S. Farrar,

Robert Palais,

Carl T. Wittwer

Publication year - 2011

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2011.166306

Subject(s) - genotyping , amplicon , genotype , microbiology and biotechnology , primer (cosmetics) , high resolution melt , melting curve analysis , biology , snapback , polymerase chain reaction , genetics , chemistry , gene , physics , organic chemistry , electrostatic discharge , quantum mechanics , voltage

Gilbert syndrome, a chronic nonhemolytic unconjugated hyperbilirubinemia, is associated with thymine-adenine (TA) insertions in the UGT1A1 (UDP glucuronosyltransferase 1 family, polypeptide A1) promoter. The UGT1A1 promoter genotype also correlates with toxicity induced by the chemotherapeutic drug irinotecan. Current closed-tube assays for genotyping the UGT1A1 (TA)(n) promoter polymorphism require multiple labeled probes and/or have difficulty classifying the (TA)(5) and (TA)(8) alleles.

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