Comparative Analysis of PCR–Electrospray Ionization/Mass Spectrometry (MS) and MALDI-TOF/MS for the Identification of Bacteria and Yeast from Positive Blood Culture Bottles | Zendy

Erin Kaleta | Zendy; Andrew E. Clark | Zendy; Abdessalam Cherkaoui | Zendy; Vicki H. Wysocki | Zendy; Elizabeth L. Ingram | Zendy; Jacques Schrenzel | Zendy; Donna M. Wolk | Zendy

Open Access

Comparative Analysis of PCR–Electrospray Ionization/Mass Spectrometry (MS) and MALDI-TOF/MS for the Identification of Bacteria and Yeast from Positive Blood Culture Bottles

Author(s) -

Erin Kaleta,

Andrew E. Clark,

Abdessalam Cherkaoui,

Vicki H. Wysocki,

Elizabeth L. Ingram,

Jacques Schrenzel,

Donna M. Wolk

Publication year - 2011

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2011.161968

Subject(s) - mass spectrometry , electrospray ionization , chromatography , matrix assisted laser desorption/ionization , blood culture , chemistry , biology , microbiology and biotechnology , antibiotics , organic chemistry , adsorption , desorption

Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR-electrospray ionization (ESI)/MS and MALDI-TOF/MS, with respect to phenotypic and biochemical profiling as a reference standard method. A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples.

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