Quantification of Serum 1–84 Parathyroid Hormone in Patients with Hyperparathyroidism by Immunocapture In Situ Digestion Liquid Chromatography–Tandem Mass Spectrometry | Zendy

Vivek Kumar | Zendy; David R. Barnidge | Zendy; Li-Sheng Chen | Zendy; Jolaine M Twentyman | Zendy; Kendall W Cradic | Zendy; Stefan K. Grebe | Zendy; Ravinder Singh | Zendy

Open Access

Quantification of Serum 1–84 Parathyroid Hormone in Patients with Hyperparathyroidism by Immunocapture In Situ Digestion Liquid Chromatography–Tandem Mass Spectrometry

Author(s) -

Vivek Kumar,

David R. Barnidge,

Li-Sheng Chen,

Jolaine M Twentyman,

Kendall W Cradic,

Stefan K. Grebe,

Ravinder Singh

Publication year - 2009

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2009.134643

Subject(s) - parathyroid hormone , immunoassay , chromatography , detection limit , chemistry , liquid chromatography–mass spectrometry , mass spectrometry , hyperparathyroidism , medicine , calcium , antibody , organic chemistry , immunology

Immunoassays specific for 1-84 parathyroid hormone (PTH) reportedly reflect the bioactivity of PTH; however, PTH immunoassays can be susceptible to interference by cross-reacting PTH fragments. In addition, these assays currently lack standardization. A methodology using immunocapture purification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection, along with a stable isotope-labeled internal standard, may help address these issues.

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