Open AccessDesign and Optimization of Reverse-Transcription Quantitative PCR Experiments
Author(s) -
Aleš Tichopád,
Rob Kitchen,
Irmgard Riedmaier,
Christiane Becker,
Anders Ståhlberg,
Mikael Kubista
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2009.126201
Subject(s) - reverse transcription polymerase chain reaction , reverse transcriptase , computational biology , biology , polymerase chain reaction , chemistry , genetics , gene , messenger rna
Quantitative PCR (qPCR) is a valuable technique for accurately and reliably profiling and quantifying gene expression. Typically, samples obtained from the organism of study have to be processed via several preparative steps before qPCR.
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