Open AccessMethod for the Selective Measurement of Amino-Terminal Variants of Procalcitonin
Author(s) -
Joachim Struck,
M. Strebelow,
Sonja Tietz,
Christine Alonso,
Nils G. Morgenthaler,
Johannes G. van der Hoeven,
Peter Pickkers,
Andreas Bergmann
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.123018
Subject(s) - procalcitonin , monoclonal antibody , epitope , antibody , amino acid , monoclonal , chemistry , radioimmunoassay , biochemistry , microbiology and biotechnology , biology , immunology , sepsis
Procalcitonin (PCT) is an established marker for diagnosing and monitoring bacterial infections. Full-length PCT [116 amino acids that make up procalcitonin (PCT1-116)] can be truncated, leading to des-Ala-Pro-PCT (des-Alanin-Prolin-Procalcitonin; PCT3-116). Current immunoassays for PCT ("total PCT") use antibodies directed against internal epitopes and are unable to distinguish amino-terminal PCT variants. Here we describe the development of monoclonal antibodies recognizing the amino-termini of PCT1-116 and PCT3-116 and their use in the selective measurement of these PCT species.
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