Open AccessRNA Extraction from Archival Formalin-Fixed Paraffin-Embedded Tissue: A Comparison of Manual, Semiautomated, and Fully Automated Purification Methods
Author(s) -
Kerstin Bohmann,
Guido Hennig,
Uwe Rogel,
Christopher Poremba,
Berit Mueller,
Péter Fritz,
Stephan Stoerkel,
Karl-L Schaefer
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.122572
Subject(s) - rna extraction , extraction (chemistry) , rna , chromatography , reproducibility , biology , microbiology and biotechnology , chemistry , gene , biochemistry
Formalin-fixed paraffin-embedded (FFPE) tumor material represents a valuable resource for the analysis of RNA-based biomarkers, both in research laboratories and in routine clinical testing. A robust and automated RNA-extraction method with a high sample throughput is required.
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