Open AccessCopy-Number Variation Genotyping of GSTT1 and GSTM1 Gene Deletions by Real-Time PCR
Author(s) -
Matthew RoseZerilli,
Sheila J. Barton,
A. John Henderson,
Seif O. Shaheen,
John W. Holloway
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.120105
Subject(s) - genotyping , copy number variation , genotype , biology , genetics , human genome , real time polymerase chain reaction , gene , genome , structural variation , replicate , gene dosage , computational biology , polymerase chain reaction , gene expression , statistics , mathematics
Structural variation in the human genome is increasingly recognized as being highly prevalent and having relevance to common human diseases. Array-based comparative genome-hybridization technology can be used to determine copy-number variation (CNV) across entire genomes, and quantitative PCR (qPCR) can be used to validate de novo variation or assays of common CNV in disease-association studies. Analysis of large qPCR data sets can be complicated and time-consuming, however.
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