The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Author(s) -
Stephen A. Bustin,
Vladimı́r Beneš,
Jeremy A. Garson,
Jan Hellemans,
Jim F. Huggett,
Mikael Kubista,
Reinhold Mueller,
Tania Nolan,
Michael W. Pfaffl,
Gregory L. Shipley,
Jo Vandesompele,
Carl T. Wittwer
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.112797
Subject(s) - real time polymerase chain reaction , computer science , computational biology , information retrieval , statistics , biology , mathematics , genetics , gene
Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments.
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