Open AccessMicrofluidics Digital PCR Reveals a Higher than Expected Fraction of Fetal DNA in Maternal Plasma
Author(s) -
Fiona M. F. Lun,
Rossa W. K. Chiu,
K.C. Allen Chan,
Tak Yeung Leung,
Tze Kin Lau,
Y. M. Dennis Lo
Publication year - 2008
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.111385
Subject(s) - digital polymerase chain reaction , dna , fetus , fraction (chemistry) , microfluidics , computational biology , polymerase chain reaction , biology , chemistry , pregnancy , genetics , chromatography , nanotechnology , gene , materials science
The precise measurement of cell-free fetal DNA in maternal plasma facilitates noninvasive prenatal diagnosis of fetal chromosomal aneuploidies and other applications. We tested the hypothesis that microfluidics digital PCR, in which individual fetal-DNA molecules are counted, could enhance the precision of measuring circulating fetal DNA.
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