Open AccessLiquid-Based Fluorescence In Situ Hybridization Assay for Detection of ERBB2 Gene Amplification in Patients with Breast Cancer3
Author(s) -
Chen-Hsiung Yeh,
William A Whitmire,
Maher Albitar
Publication year - 2008
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.107607
Subject(s) - fluorescence in situ hybridization , concordance , microbiology and biotechnology , immunohistochemistry , flow cytometry , streptavidin , in situ hybridization , biology , fish <actinopterygii> , breast cancer , pathology , cancer , gene , chromosome , gene expression , medicine , biotin , immunology , genetics , fishery
Current reference methods for evaluating gene amplification and expression of ERBB2 (also known as HER-2)--cell-based fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC)--are subjective and influenced by methods of tissue preparation and fixation. We developed and evaluated a novel, quantitative liquid-based FISH (L-FISH) assay that uses flow cytometry to detect ERBB2 gene amplification in breast cancer patients.
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