Open AccessNovel Immunoassay for Quantification of Brain Natriuretic Peptide and Its Precursor in Human Blood
Author(s) -
Natalia N Tamm,
Karina R Seferian,
Alexander G Semenov,
Kadriya S Mukharyamova,
Ekaterina V Koshkina,
Mihail I Krasnoselsky,
Alexander B. Postnikov,
Daria V. Serebryanaya,
Fred S. Apple,
MaryAnn M Murakami,
Alexey G Katrukha
Publication year - 2008
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2007.100545
Subject(s) - epitope , immunoassay , monoclonal antibody , antibody , natriuretic peptide , antigen , proteolysis , immune system , peptide , brain natriuretic peptide , chemistry , microbiology and biotechnology , medicine , immunology , biochemistry , heart failure , biology , enzyme
Brain natriuretic peptide (BNP) is an unstable molecule that can rapidly lose immunologic activity in blood. Conventional sandwich BNP immunoassays use 2 antibodies specific to 2 different epitopes. Larger distances between epitopes are associated with a greater probability of proteolysis sites being located between the antibody-binding sites, and thus such assays have an increased susceptibility to underdetect BNP because of the increased likelihood of proteolytic degradation. The purpose of our study was to develop a sandwich immunoassay for the precise quantification of BNP and BNP precursor (proBNP) in human blood that is not susceptible to proteolysis.
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