Measurement of Folate in Fresh and Archival Serum Samples as p-Aminobenzoylglutamate Equivalents | Zendy

Rita Hannisdal | Zendy; Asbjørn Svardal | Zendy; Per Magne Ueland | Zendy

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Measurement of Folate in Fresh and Archival Serum Samples as p-Aminobenzoylglutamate Equivalents

Author(s) -

Rita Hannisdal,

Asbjørn Svardal,

Per Magne Ueland

Publication year - 2008

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2007.100511

Subject(s) - chromatography , chemistry , hydrolysis , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , detection limit , tandem mass spectrometry , mass spectrometry , sample preparation , folic acid , biochemistry , medicine

The development of accurate and precise folate assays has been difficult, mainly because of folate instability. Large interassay and interlaboratory differences have been reported. We therefore developed a serum folate assay that measures folate and putative degradation products as p-aminobenzoylglutamate (pABG) equivalents following oxidation and acid hydrolysis.

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