MS-FLAG, a Novel Real-Time Signal Generation Method for Methylation-Specific PCR | Zendy

Cinzia Bonanno | Zendy; Erlet Shehi | Zendy; Daniel Adlerstein | Zendy; G. Mike Makrigiorgos | Zendy

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MS-FLAG, a Novel Real-Time Signal Generation Method for Methylation-Specific PCR

Author(s) -

Cinzia Bonanno,

Erlet Shehi,

Daniel Adlerstein,

G. Mike Makrigiorgos

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2007.094011

Subject(s) - bisulfite sequencing , methylation , bisulfite , dna methylation , microbiology and biotechnology , biology , amplicon , illumina methylation assay , promoter , real time polymerase chain reaction , gene , polymerase chain reaction , genetics , gene expression

Aberrant promoter methylation is a major mechanism for silencing tumor suppressor genes in cancer. Detection of hypermethylation is used as a molecular marker for early cancer diagnosis, as a prognostic index, or to define therapeutic targets for reversion of aberrant methylation. We report on a novel signal generation technology for real-time PCR to detect gene promoter methylation.

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