High-Resolution Melting Analysis of the spa Repeat Region of Staphylococcus aureus | Zendy

Alex J. Stephens | Zendy; John Inman-Bamber | Zendy; Philip M. Giffard | Zendy; Flavia Huygens | Zendy

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High-Resolution Melting Analysis of the spa Repeat Region of Staphylococcus aureus

Author(s) -

Alex J. Stephens,

John Inman-Bamber,

Philip M. Giffard,

Flavia Huygens

Publication year - 2008

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2007.093658

Subject(s) - genotyping , locus (genetics) , staphylococcus aureus , repeatability , melting curve analysis , high resolution melt , biology , genetics , gene , computational biology , genotype , polymerase chain reaction , chemistry , chromatography , bacteria

The staphylococcal protein A (spa) locus of Staphylococcus aureus contains a complex repeat structure and is commonly used for single-locus sequence-based genotyping. The real-time PCR platform supports genotyping methods that are single step and closed tube and potentially can be carried out simultaneously with diagnosis. We describe here a method for genotyping S. aureus using high-resolution melting (HRM) analysis of the spa polymorphic region X.

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