Open AccessUnlabeled Probes for the Detection and Typing of Herpes Simplex Virus
Author(s) -
Shale Dames,
David C. Pattison,
L Kathryn Bromley,
Carl T. Wittwer,
Karl V. Voelkerding
Publication year - 2007
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2007.090761
Subject(s) - amplicon , typing , herpes simplex virus , hybridization probe , microbiology and biotechnology , genotyping , biology , molecular probe , digoxigenin , polymerase chain reaction , dna , virology , genotype , chemistry , virus , genetics , gene , in situ hybridization , gene expression
Unlabeled probe detection with a double-stranded DNA (dsDNA) binding dye is one method to detect and confirm target amplification after PCR. Unlabeled probes and amplicon melting have been used to detect small deletions and single-nucleotide polymorphisms in assays where template is in abundance. Unlabeled probes have not been applied to low-level target detection, however.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom