EQUAL-qual: A European Program for External Quality Assessment of Genomic DNA Extraction and PCR Amplification | Zendy

Claudio Orlando | Zendy; Paolo Verderio | Zendy; R. Maatman | Zendy; Jan Danneberg | Zendy; Simon Ramsden | Zendy; Michael Neumaier | Zendy; Domenica Taruscio | Zendy; Vincenzo Falbo | Zendy; Robert K. Jansen | Zendy; C. Casini-Raggi | Zendy; Francesca Malentacchi | Zendy; Ettore Marubini | Zendy; Sara Pizzamiglio | Zendy; Kris Vernelen | Zendy; Jean-Claude Libeer | Zendy; Vladimír Palička | Zendy; Mario Pazzagli | Zendy

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EQUAL-qual: A European Program for External Quality Assessment of Genomic DNA Extraction and PCR Amplification

Author(s) -

Claudio Orlando,

Paolo Verderio,

R. Maatman,

Jan Danneberg,

Simon Ramsden,

Michael Neumaier,

Domenica Taruscio,

Vincenzo Falbo,

Robert K. Jansen,

C. Casini-Raggi,

Francesca Malentacchi,

Ettore Marubini,

Sara Pizzamiglio,

Kris Vernelen,

Jean-Claude Libeer,

Vladimír Palička,

Mario Pazzagli

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.084004

Subject(s) - external quality assessment , dna extraction , quality assessment , primer (cosmetics) , computational biology , polymerase chain reaction , chromatography , extraction (chemistry) , medical physics , computer science , biology , statistics , medicine , chemistry , mathematics , genetics , pathology , gene , organic chemistry

Despite the rapid transition into routine clinical practice of molecular techniques based on PCR, external quality assessment (EQA) is still not widely available. The European Union and European Communities Confederation of Clinical Chemistry have supported the EQUAL project as a series of 3 different EQA programs for the assessment of molecular methods independently from analytes. We present the results from the EQUAL-qual program designed to evaluate the analytical aspects of DNA analysis by means of a conventional qualitative PCR experiment.

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