Evaluation of a New Pooling Strategy Based on Leukocyte Count for Rapid Quantification of Allele Frequencies | Zendy

Heidi Rossmann | Zendy; Elena Büchler | Zendy; Jürgen J. Wenzel | Zendy; Carolin Neukirch | Zendy; JeanBaptist du Prel | Zendy; Karl J. Lackner | Zendy

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Evaluation of a New Pooling Strategy Based on Leukocyte Count for Rapid Quantification of Allele Frequencies

Author(s) -

Heidi Rossmann,

Elena Büchler,

Jürgen J. Wenzel,

Carolin Neukirch,

JeanBaptist du Prel,

Karl J. Lackner

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.083691

Subject(s) - pooling , single nucleotide polymorphism , allele , genotype , dna , allele frequency , pyrosequencing , biology , dna extraction , microbiology and biotechnology , snp , genetics , polymerase chain reaction , gene , computer science , artificial intelligence

Allele frequencies of single-nucleotide polymorphisms (SNPs) can be quantified from DNA pools. The conventional preparation of DNA pools requires DNA isolation and quantification for each blood sample. We hypothesized that pooling of whole blood samples according to their leukocyte count, which determines DNA content, would be as reliable as the conventional pooling method but much less tedious to perform.

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