Chronic Granulomatous Disease (CGD) and Complete Myeloperoxidase Deficiency Both Yield Strongly Reduced Dihydrorhodamine 123 Test Signals but Can Be Easily Discerned in Routine Testing for CGD | Zendy

Lysann Mauch | Zendy; Andreas Lun | Zendy; Maurice R.G. O’Gorman | Zendy; John S. Harris | Zendy; Ilka Schulze | Zendy; Arturo Zychlinsky | Zendy; Tobias A. Fuchs | Zendy; Uta Oelschlägel | Zendy; Sebastian Brenner | Zendy; D Kutter | Zendy; Angela RösenWolff | Zendy; Joachim Roesler | Zendy

Open Access

Chronic Granulomatous Disease (CGD) and Complete Myeloperoxidase Deficiency Both Yield Strongly Reduced Dihydrorhodamine 123 Test Signals but Can Be Easily Discerned in Routine Testing for CGD

Author(s) -

Lysann Mauch,

Andreas Lun,

Maurice R.G. O’Gorman,

John S. Harris,

Ilka Schulze,

Arturo Zychlinsky,

Tobias A. Fuchs,

Uta Oelschlägel,

Sebastian Brenner,

D Kutter,

Angela RösenWolff,

Joachim Roesler

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.083444

Subject(s) - chronic granulomatous disease , myeloperoxidase , nadph oxidase , lucigenin , immunology , peroxidase , microbiology and biotechnology , chemistry , medicine , biochemistry , enzyme , biology , inflammation , superoxide

The flow cytometric dihydrorhodamine 123 (DHR) assay is used as a screening test for chronic granulomatous disease (CGD), but complete myeloperoxidase (MPO) deficiency can also lead to a strongly decreased DHR signal. Our aim was to devise simple laboratory methods to differentiate MPO deficiency (false positive for CGD) and NADPH oxidase abnormalities (true CGD).

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