HPLC for Simultaneous Quantification of Total Ceramide, Glucosylceramide, and Ceramide Trihexoside Concentrations in Plasma | Zendy

J.E.M. Groener | Zendy; Ben J. H. M. Poorthuis | Zendy; Sijmen Kuiper | Zendy; Mariette T.J. Helmond | Zendy; Carla E. M. Hollak | Zendy; Johannes M. F. G. Aerts | Zendy

Open Access

HPLC for Simultaneous Quantification of Total Ceramide, Glucosylceramide, and Ceramide Trihexoside Concentrations in Plasma

Author(s) -

J.E.M. Groener,

Ben J. H. M. Poorthuis,

Sijmen Kuiper,

Mariette T.J. Helmond,

Carla E. M. Hollak,

Johannes M. F. G. Aerts

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.079012

Subject(s) - ceramide , chromatography , chemistry , high performance liquid chromatography , derivatization , sphingolipid , detection limit , methanol , fluorescence spectroscopy , fluorescence , biochemistry , organic chemistry , apoptosis , physics , quantum mechanics

Simple, reproducible assays are needed for the quantification of sphingolipids, ceramide (Cer), and sphingoid bases. We developed an HPLC method for simultaneous quantification of total plasma concentrations of Cer, glucosylceramide (GlcCer), and ceramide trihexoside (CTH).

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