Effects of Storage, RNA Extraction, Genechip Type, and Donor Sex on Gene Expression Profiling of Human Whole Blood | Zendy

SungJae Kim | Zendy; David J. Dix | Zendy; Kary E. Thompson | Zendy; Rachel N. Murrell | Zendy; Judith E. Schmid | Zendy; Jane Gallagher | Zendy; John C Rockett | Zendy

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Effects of Storage, RNA Extraction, Genechip Type, and Donor Sex on Gene Expression Profiling of Human Whole Blood

Author(s) -

SungJae Kim,

David J. Dix,

Kary E. Thompson,

Rachel N. Murrell,

Judith E. Schmid,

Jane Gallagher,

John C Rockett

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.078436

Subject(s) - rna extraction , gene chip analysis , rna , gene expression , gene expression profiling , whole blood , biology , microarray , microarray analysis techniques , gene , genetics , immunology

Gene expression profiling of whole blood may be useful for monitoring toxicological exposure and for diagnosis and monitoring of various diseases. Several methods are available that can be used to transport, store, and extract RNA from whole blood, but it is not clear which procedures alter results. In addition, characterization of interindividual and sex-based variation in gene expression is needed to understand sources and extent of variability.

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