Standardized Peptidome Profiling of Human Urine by Magnetic Bead Separation and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry | Zendy

Martin Fiedler | Zendy; Sven Baumann | Zendy; Alexander Leichtle | Zendy; Anke Oltmann | Zendy; Julia Kase | Zendy; Joachim Thiery | Zendy; Uta Ceglarek | Zendy

Open Access

Standardized Peptidome Profiling of Human Urine by Magnetic Bead Separation and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Author(s) -

Martin Fiedler,

Sven Baumann,

Alexander Leichtle,

Anke Oltmann,

Julia Kase,

Joachim Thiery,

Uta Ceglarek

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.077834

Subject(s) - urine , chemistry , mass spectrometry , chromatography , ion suppression in liquid chromatography–mass spectrometry , time of flight mass spectrometry , urinary system , tandem mass spectrometry , ionization , ion , biochemistry , medicine , organic chemistry

Peptidome profiling of human urine is a promising tool to identify novel disease-associated biomarkers; however, a wide range of preanalytical variables influence the results of peptidome analysis. Our aim was to develop a standardized protocol for reproducible urine peptidome profiling by means of magnetic bead (MB) separation followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS).

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