High-Throughput Microtiter Well-Based Chemiluminometric Genotyping of 15 HBB Gene Mutations in a Dry-Reagent Format | Zendy

Kyriaki Glynou | Zendy; Petros Kastanis | Zendy; Sotiria Boukouvala | Zendy; Vassilis Tsaoussis | Zendy; Penelope C. Ioannou | Zendy; Theodore K. Christopoulos | Zendy; Joanne TraegerSynodinos | Zendy; Emmanuel Kanavakis | Zendy

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High-Throughput Microtiter Well-Based Chemiluminometric Genotyping of 15 HBB Gene Mutations in a Dry-Reagent Format

Author(s) -

Kyriaki Glynou,

Petros Kastanis,

Sotiria Boukouvala,

Vassilis Tsaoussis,

Penelope C. Ioannou,

Theodore K. Christopoulos,

Joanne TraegerSynodinos,

Emmanuel Kanavakis

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.077776

Subject(s) - genotyping , microbiology and biotechnology , chemiluminescence , genotype , reagent , mutation , mutant , biology , chemistry , gene , chromatography , genetics

Hemoglobinopathies are the most common inherited diseases worldwide. Various methods for genotyping of hemoglobin, beta (HBB) gene mutations have been reported, but there is need for a high sample-throughput, cost-effective method for simultaneous screening of several mutations. We report a method that combines the high detectability and dynamic range of chemiluminescence with the high allele-discrimination ability of probe extension reactions for simultaneous genotyping of 15 HBB mutations in a high sample-throughput, dry-reagent format.

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