Open AccessEvaluation of a 1,25-Dihydroxyvitamin D Enzyme Immunoassay
Author(s) -
Isolde SeidenLong,
Reinhold Vieth
Publication year - 2007
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2006.077560
Subject(s) - immunoassay , chromatography , enzyme , chemistry , medicine , biochemistry , antibody , immunology
Radioactive reagents are used in most assays for measurement of 1,25-dihydroxyvitamin D [1,25(OH)(2)D]. We evaluated a 1,25(OH)(2)D enzyme immunoassay (EIA) from IDS Ltd. that uses solid-phase immunoextraction and colorimetric detection and compared results to those of the thymus radioreceptor assay (RRA) for 1,25(OH)(2)D.
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