Real-time PCR Assay for Ultrasensitive Quantification of DNA-Binding Proteins | Zendy

Peng Hou | Zendy; Zaozao Chen | Zendy; Meiju Ji | Zendy; gyue He | Zendy; Zuhong Lu | Zendy

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Real-time PCR Assay for Ultrasensitive Quantification of DNA-Binding Proteins

Author(s) -

Peng Hou,

Zaozao Chen,

Meiju Ji,

gyue He,

Zuhong Lu

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.077503

Subject(s) - dna binding site , dna , microbiology and biotechnology , exonuclease iii , dna binding protein , dna footprinting , biology , exonuclease , hmg box , binding site , primer (cosmetics) , chemistry , transcription factor , dna polymerase , biochemistry , gene , gene expression , promoter , escherichia coli , organic chemistry

The specific binding of proteins to DNA is a key step for many cellular activities, such as transcription regulation, DNA replication, recombination, repair, and restriction. The detection of DNA-binding proteins, as well as the identification of specific binding sites, is therefore important to understand gene expression mechanisms and cellular function. We describe an ultrasensitive method for quantification of DNA-binding proteins.

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