Molecular Beacon–Based Temperature Control and Automated Analyses for Improved Resolution of Melting Temperature Analysis Using SYBR I Green Chemistry | Zendy

Christoffer Nellåker | Zendy; Ulf Wållgren | Zendy; Håkan Karlsson | Zendy

Open Access

Molecular Beacon–Based Temperature Control and Automated Analyses for Improved Resolution of Melting Temperature Analysis Using SYBR I Green Chemistry

Author(s) -

Christoffer Nellåker,

Ulf Wållgren,

Håkan Karlsson

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.075184

Subject(s) - melting curve analysis , melting temperature , chemistry , analytical chemistry (journal) , temperature control , dissociation (chemistry) , sybr green i , temperature measurement , thermodynamics , materials science , real time polymerase chain reaction , chromatography , physics , biochemistry , composite material , gene

Melting temperature analysis of products amplified with SYBR I Green chemistry is a cheap and effective method for identification of sequence differences. When used in conventional quantitative real-time PCR instruments (qPCR), this method is limited by temperature variations over the heating block and low numbers of fluorescence measurements during the dissociation step, which hamper the ability of most instruments to report accurate and precise melting temperatures.

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