HPLC-Electrospray Tandem Mass Spectrometry for Rapid Determination of Dihydropyrimidine Dehydrogenase Activity | Zendy

André B. P. Kuilenburg | Zendy; Henk van Lenthe | Zendy; Lida Zoetekouw | Zendy; Willem Kulik | Zendy

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HPLC-Electrospray Tandem Mass Spectrometry for Rapid Determination of Dihydropyrimidine Dehydrogenase Activity

Author(s) -

André B. P. Kuilenburg,

Henk van Lenthe,

Lida Zoetekouw,

Willem Kulik

Publication year - 2007

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.074096

Subject(s) - chemistry , high performance liquid chromatography , chromatography , tandem mass spectrometry , selected reaction monitoring , electrospray ionization , dihydropyrimidine dehydrogenase , detection limit , mass spectrometry , thymine , biochemistry , fluorouracil , dna , medicine , surgery , chemotherapy , thymidylate synthase

Patients with a partial dihydropyrimidine dehydrogenase (DPD) deficiency have an increased risk of developing severe 5-fluorouracil-associated toxicity. We developed a rapid and specific method to measure the DPD activity in peripheral blood mononuclear cells using HPLC tandem-mass spectrometry (HPLC-MS/MS).

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