Targeted Gene-Expression Analysis by Genome-Controlled Reverse Transcription-PCR | Zendy

Jakob Stenman | Zendy; Annukka Paju | Zendy; Oso Rissanen | Zendy; Tuomas Tenkanen | Zendy; Caj Haglund | Zendy; Jari Räsänen | Zendy; Jarmo Salo | Zendy; UlfHåkan Stenman | Zendy; Arto Orpana | Zendy

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Targeted Gene-Expression Analysis by Genome-Controlled Reverse Transcription-PCR

Author(s) -

Jakob Stenman,

Annukka Paju,

Oso Rissanen,

Tuomas Tenkanen,

Caj Haglund,

Jari Räsänen,

Jarmo Salo,

UlfHåkan Stenman,

Arto Orpana

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.073064

Subject(s) - amplicon , taqman , microbiology and biotechnology , melting curve analysis , gene expression , reproducibility , biology , complementary dna , reverse transcription polymerase chain reaction , real time polymerase chain reaction , gene , biopsy , polymerase chain reaction , pathology , chemistry , medicine , genetics , chromatography

For gene-expression analysis, which is anticipated to play an important role in classification of tumors and premalignant conditions, PCR-based quantitative assays must have increased diagnostic quantitative accuracy and reproducibility and enable analysis of gene expression in formalin-fixed paraffin-embedded (FFPE) tissue samples.

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