High-Volume Extraction of Nucleic Acids by Magnetic Bead Technology for Ultrasensitive Detection of Bacteria in Blood Components | Zendy

Melanie Störmer | Zendy; K Kleesiek | Zendy; J Dreier | Zendy

Open Access

High-Volume Extraction of Nucleic Acids by Magnetic Bead Technology for Ultrasensitive Detection of Bacteria in Blood Components

Author(s) -

Melanie Störmer,

K Kleesiek,

J Dreier

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.070987

Subject(s) - nucleic acid , detection limit , chromatography , staphylococcus epidermidis , nucleic acid methods , primer (cosmetics) , bacteria , dna extraction , chemistry , biology , polymerase chain reaction , biochemistry , staphylococcus aureus , organic chemistry , gene , genetics

Nucleic acid isolation, the most technically demanding and laborious procedure performed in molecular diagnostics, harbors the potential for improvements in automation. A recent development is the use of magnetic beads covered with nucleic acid-binding matrices. We adapted this technology with a broad-range 23S rRNA real-time reverse transcription (RT)-PCR assay for fast and sensitive detection of bacterial contamination of blood products.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research