Use of Frozen Sera for FT4 Standardization: Investigation by Equilibrium Dialysis Combined with Isotope Dilution-Mass Spectrometry and Immunoassay | Zendy

Katleen Van Uytfanghe | Zendy; Dietmar Stöckl | Zendy; H A Ross | Zendy; Linda M. Thienpont | Zendy

Open Access

Use of Frozen Sera for FT4 Standardization: Investigation by Equilibrium Dialysis Combined with Isotope Dilution-Mass Spectrometry and Immunoassay

Author(s) -

Katleen Van Uytfanghe,

Dietmar Stöckl,

H A Ross,

Linda M. Thienpont

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2006.070425

Subject(s) - chromatography , isotope dilution , detection limit , immunoassay , chemistry , mass spectrometry , dialysis , tandem mass spectrometry , liquid chromatography–mass spectrometry , extraction (chemistry) , certified reference materials , dilution , medicine , antibody , immunology , physics , thermodynamics

Serum-free thyroxine (FT4) testing is recommended for diagnosis or monitoring of thyroid dysfunction, particularly in cases of hormone binding abnormalities. However, the poor intermethod agreement among commercial FT4 assays suggests a need for standardization with a hierarchically higher measurement procedure. To that purpose, we applied equilibrium dialysis (ED) in combination with isotope dilution-liquid chromatography/tandem mass spectrometry (ID-LC-tandem MS).

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