EQUAL-quant: An International External Quality Assessment Scheme for Real-Time PCR | Zendy

Simon Ramsden | Zendy; Sarah B. Daly | Zendy; WolfJochen Geilenkeuser | Zendy; Graeme Duncan | Zendy; Fabienne Hermitte | Zendy; Ettore Marubini | Zendy; Michael Neumaier | Zendy; Claudio Orlando | Zendy; Vladimír Palička | Zendy; Angelo Paradiso | Zendy; Mario Pazzagli | Zendy; Sara Pizzamiglio | Zendy; Paolo Verderio | Zendy

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EQUAL-quant: An International External Quality Assessment Scheme for Real-Time PCR

Author(s) -

Simon Ramsden,

Sarah B. Daly,

WolfJochen Geilenkeuser,

Graeme Duncan,

Fabienne Hermitte,

Ettore Marubini,

Michael Neumaier,

Claudio Orlando,

Vladimír Palička,

Angelo Paradiso,

Mario Pazzagli,

Sara Pizzamiglio,

Paolo Verderio

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2005.066019

Subject(s) - external quality assessment , quality assurance , taqman , medical physics , computer science , european union , data mining , real time polymerase chain reaction , medicine , biology , pathology , business , economic policy , biochemistry , gene

Quantitative gene expression analysis by real-time PCR is important in several diagnostic areas, such as the detection of minimum residual disease in leukemia and the prognostic assessment of cancer patients. To address quality assurance in this technically challenging area, the European Union (EU) has funded the EQUAL project to develop methodologic external quality assessment (EQA) relevant to diagnostic and research laboratories among the EU member states. We report here the results of the EQUAL-quant program, which assesses standards in the use of TaqMan probes, one of the most widely used assays in the implementation of real-time PCR.

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