Sensitive and Quantitative Measurement of Gene Expression Directly from a Small Amount of Whole Blood | Zendy

Zhi Zheng | Zendy; Yuling Luo | Zendy; Gary McMaster | Zendy

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Sensitive and Quantitative Measurement of Gene Expression Directly from a Small Amount of Whole Blood

Author(s) -

Zhi Zheng,

Yuling Luo,

Gary McMaster

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2005.065078

Subject(s) - multiplex , whole blood , microbiology and biotechnology , gene expression , real time polymerase chain reaction , biology , lysis , messenger rna , lysis buffer , gene , multiplex polymerase chain reaction , dna , chemistry , polymerase chain reaction , biochemistry , immunology , genetics

Accurate and precise quantification of mRNA in whole blood is made difficult by gene expression changes during blood processing, and by variations and biases introduced by sample preparations. We sought to develop a quantitative whole-blood mRNA assay that eliminates blood purification, RNA isolation, reverse transcription, and target amplification while providing high-quality data in an easy assay format.

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