Systematic Comparison of the T7-IVT and SMART-Based RNA Preamplification Techniques for DNA Microarray Experiments | Zendy

Jochen Wilhelm | Zendy; Jai Prakash Muyal | Zendy; Johannes Best | Zendy; Grażyna Kwapiszewska | Zendy; Maria Magdalena Stein | Zendy; Werner Seeger | Zendy; Rainer M. Bohle | Zendy; Ludger Fink | Zendy

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Systematic Comparison of the T7-IVT and SMART-Based RNA Preamplification Techniques for DNA Microarray Experiments

Author(s) -

Jochen Wilhelm,

Jai Prakash Muyal,

Johannes Best,

Grażyna Kwapiszewska,

Maria Magdalena Stein,

Werner Seeger,

Rainer M. Bohle,

Ludger Fink

Publication year - 2006

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2005.062406

Subject(s) - microarray , microbiology and biotechnology , laser capture microdissection , dna microarray , biology , rna , microarray analysis techniques , gene expression , computational biology , gene , genetics

Small biological samples obtained from biopsies or laser microdissection often do not yield sufficient RNA for successful microarray hybridization; therefore, RNA amplification is performed before microarray experiments. We compared 2 commonly used techniques for RNA amplification.

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