Open AccessAssay for Measurement of Intact B-Type Natriuretic Peptide Prohormone in Blood
Author(s) -
Isabelle Giuliani,
François Rieunier,
Catherine Larue,
J.F. Delagneau,
Claude Granier,
Bernard Pau,
M Ferrière,
M. Saussine,
JeanPaul Cristol,
AnneMarie Dupuy,
Emmanuel Y. Mérigeon,
Delphine Merle,
Sylvie Villard
Publication year - 2006
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2005.061770
Subject(s) - prohormone , immunoassay , natriuretic peptide , polyclonal antibodies , monoclonal antibody , recombinant dna , epitope , cleavage (geology) , peptide , chemistry , antibody , brain natriuretic peptide , microbiology and biotechnology , medicine , endocrinology , heart failure , biochemistry , biology , immunology , hormone , gene , paleontology , fracture (geology)
B-Type natriuretic peptide (BNP1-32) as well as the N-terminal fragment of the prohormone containing residues 1-76 (NT-proBNP1-76), both cleavage products of the precursor proBNP1-108, are reported to be powerful markers for prognosis and risk stratification of heart failure. However, the intact precursor also circulates in the bloodstream. Assays for the detection of these cleavage products have been developed, but most of these assays may overestimate the concentrations of the cleavage products because they also measure the precursor form. It is therefore important to develop an immunoassay that specifically measures solely proBNP1-108 in plasma.
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