High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in Solution | Zendy

Luming Zhou | Zendy; Lesi Wang | Zendy; Robert Palais | Zendy; Robert J. Pryor | Zendy; Carl T. Wittwer | Zendy

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High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in Solution

Author(s) -

Luming Zhou,

Lesi Wang,

Robert Palais,

Robert J. Pryor,

Carl T. Wittwer

Publication year - 2005

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2005.054924

Subject(s) - high resolution melt , melting curve analysis , genotyping , microbiology and biotechnology , oligonucleotide , biology , polymerase chain reaction , genotype , dna , molecular inversion probe , primer dimer , chemistry , genetics , gene , multiplex polymerase chain reaction

High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described.

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