Real-Time PCR Assays Targeting a Unique Chromosomal Sequence of Yersinia pestis
Author(s) -
C. J. Chase,
Melanie Ulrich,
Leonard P. Wasieloski,
John P. Kondig,
Jeffrey Garrison,
Luther E. Lindler,
David A. Kulesh
Publication year - 2005
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2005.051839
Subject(s) - yersinia pestis , yersinia pseudotuberculosis , biology , plasmid , polymerase chain reaction , gene , hybridization probe , microbiology and biotechnology , genetics , virulence
Yersinia pestis, the causative agent of the zoonotic infection plague, is a major concern as a potential bioweapon. Current real-time PCR assays used for Y. pestis detection are based on plasmid targets, some of which may generate false-positive results.
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